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1 Comparison of \(g\) groups

  • Extend \(F\)-test from a one-way ANOVA to non-parametric alternatives.

2 DMH Voorbeeld

Assess genotoxicity of 1,2-dimethylhydrazine dihydrochloride (DMH) (EU directive)

  • 24 rats
  • four groups with daily DMH dose
    • control
    • low
    • medium
    • high
  • Genotoxicity in liver using comet assay on 150 liver cells per rat.
  • Are there differences in DNA damage due to DMH dose?

2.1 Comet Assay:

  • Visualise DNA strand breaks
  • Length comet tail is a proxy for strand breaks.

Comet assay

dna <- read_delim("https://raw.githubusercontent.com/GTPB/PSLS20/master/data/dna.txt",delim=" ")
dna$dose <- as.factor(dna$dose)
dna
dna %>% 
  ggplot(aes(x=dose,y=length,fill=dose))+
  geom_boxplot() +
  geom_point(position="jitter")

dna %>%
  ggplot(aes(sample=length)) +
  geom_qq() + 
  geom_qq_line() +
  facet_wrap(~dose)

  • Strong indication that data in control group has a lower variance.
  • 6 observations per group are too few to check the assumptions
plot(lm(length~dose,data=dna))

3 Kruskal-Wallis Rank Test

  • The Kruskal-Wallis Rank Test (KW-test) is a non-parameteric alternative for ANOVA F-test.

  • Classical \(F\)-teststatistic can be written as \[ F = \frac{\text{SST}/(g-1)}{\text{SSE}/(n-g)} = \frac{\text{SST}/(g-1)}{(\text{SSTot}-\text{SST})/(n-g)} , \]

  • with \(g\) the number of groups.

  • SSTot depends only on outcomes \(\mathbf{y}\) and will not vary in permutation test.

  • SST can be used as statistic \[\text{SST}=\sum_{j=1}^t n_j(\bar{Y}_j-\bar{Y})^2\]

  • The KW test statistic is based on SST on rank-transformed outcomes1, \[ \text{SST} = \sum_{j=1}^g n_j \left(\bar{R}_j - \bar{R}\right)^2 = \sum_{j=1}^t n_j \left(\bar{R}_j - \frac{n+1}{2}\right)^2 , \]

  • with \(\bar{R}_j\) the mean of the ranks in group \(j\), and \(\bar{R}\) the mean of all ranks, \[ \bar{R} = \frac{1}{n}(1+2+\cdots + n) = \frac{1}{n}\frac{1}{2}n(n+1) = \frac{n+1}{2}. \]

  • The KW teststatistic is given by \[ KW = \frac{12}{n(n+1)} \sum_{j=1}^g n_j \left(\bar{R}_j - \frac{n+1}{2}\right)^2. \]

  • The factor \(\frac{12}{n(n+1)}\) is used so that \(KW\) has a simple asymptotic null distribution. In particular under \(H_0\), given thart \(\min(n_1,\ldots, n_g)\rightarrow \infty\), \[ KW \rightarrow \chi^2_{t-1}. \]

  • The exact KW-test can be executed by calculating the permutation null distribution (that only depends on \(n_1, \ldots, n_g\)) to test \[H_0: f_1=\ldots=f_g \text{ vs } H_1: \text{ at least two means are different}.\]

  • In order to allow \(H_1\) to be formulated in terms of means, the assumption of locations shift should be valid.

  • For DMH example this is not the case.

  • If location-shift is invalid, we have to formulate \(H_1\) in terms of probabilistic indices: \[H_0: f_1=\ldots=f_g \text{ vs } H_1: \exists\ j,k \in \{1,\ldots,g\} : \text{P}\left[Y_j\geq Y_k\right]\neq 0.5\]

3.1 DNA Damage Example

kruskal.test(length~dose,data=dna)

    Kruskal-Wallis rank sum test

data:  length by dose
Kruskal-Wallis chi-squared = 14, df = 3, p-value = 0.002905
  • On the \(5\%\) level of significance we can reject the null hypothesis.

  • R-functie kruskal.test only returns the asymptotic approximation for \(p\)-values.

  • With only 6 observaties per groep, this is not a good approximation of the \(p\)-value

  • With the coin R package we can calculate the exacte \(p\)-value

library(coin)
kwPerm <- kruskal_test(length~dose,data=dna,
            distribution=approximate(B=100000))
kwPerm

    Approximative Kruskal-Wallis Test

data:  length by dose (0, 1.25, 2.5, 5)
chi-squared = 14, p-value = 0.00043
  • We conclude that the difference in the distribution of the DNA damages due to the DMH dose is extremely significantly different.

  • Posthoc analysis with WMW tests.

pairwise.wilcox.test(dna$length,dna$dose)

    Pairwise comparisons using Wilcoxon rank sum exact test 

data:  dna$length and dna$dose 

     0     1.25  2.5  
1.25 0.013 -     -    
2.5  0.013 0.818 -    
5    0.013 0.721 0.788

P value adjustment method: holm 
  • All DMH behandelingen are significantly different from the control.
  • The DMH are not significantly different from one another.
  • U1 does not occur in the pairwise.wilcox.test output. Point estimate on probability on higher DNA-damage?
nGroup <- table(dna$dose)
probInd <- combn(levels(dna$dose),2,function(x) 
         {
         test <- wilcox.test(length~dose,subset(dna,dose%in%x))
         return(test$statistic/prod(nGroup[x]))
         }
         )
names(probInd) <- combn(levels(dna$dose),2,paste,collapse="vs")
probInd
  0vs1.25    0vs2.5      0vs5 1.25vs2.5   1.25vs5    2.5vs5 
0.0000000 0.0000000 0.0000000 0.4444444 0.2777778 0.3333333 

Because there are doubts on the location-shift model we draw our conclusions in terms of the probabilistic index.

3.1.1 Conclusion

  • There is an extremely significant difference in in the distribution of the DNA-damage measurements due to the treatment with DMH (\(p<0.001\) KW-test).
  • DNA-damage is more likely upon DMH treatment than in the control treatment (all p=0.013, WMW-testen).
  • The probability on higher DNA-damage upon exposure to DMH is 100% (Calculation of a CI on the probabilistic index is beyond the scope of the course)
  • There are no significant differences in the distributions of the comit-lengths among the treatment with the different DMH concentrations (\(p=\) 0.72-0.82).
  • DMH shows already genotoxic effects at low dose.
  • (Alle paarswise tests are gecorrected for multiple testing using Holm’s methode).

  1. we assume that no ties are available↩︎

---
title: "9. Non-parametric statistics - Kruskal Wallis"  
author: "Lieven Clement"
date: "statOmics, Ghent University (https://statomics.github.io)"
output:
    html_document:
      code_download: true    
      theme: cosmo
      toc: true
      toc_float: true
      highlight: tango
      number_sections: true
---

<a rel="license" href="https://creativecommons.org/licenses/by-nc-sa/4.0"><img alt="Creative Commons License" style="border-width:0" src="https://i.creativecommons.org/l/by-nc-sa/4.0/88x31.png" /></a>

```{r setup, include=FALSE}
knitr::opts_chunk$set(include = TRUE, comment = NA, echo = TRUE,
                      message = FALSE, warning = FALSE)
library(tidyverse)
set.seed(140)
```


# Comparison of $g$ groups

- Extend  $F$-test from a one-way ANOVA to non-parametric alternatives.

# DMH Voorbeeld

Assess genotoxicity of 1,2-dimethylhydrazine dihydrochloride (DMH)  (EU directive)

- 24 rats 
- four groups with daily DMH dose
  - control
  - low
  - medium 
  - high

- Genotoxicity in liver using comet assay on 150 liver cells per rat.
- Are there differences in DNA damage due to DMH dose?

## Comet Assay:

- Visualise DNA strand breaks
- Length comet tail is a proxy for strand breaks. 

![Comet assay](https://raw.githubusercontent.com/GTPB/PSLS20/gh-pages/assets/figs/comet.jpg){ width=50% }


```{r}
dna <- read_delim("https://raw.githubusercontent.com/GTPB/PSLS20/master/data/dna.txt",delim=" ")
dna$dose <- as.factor(dna$dose)
dna
```


```{r}
dna %>% 
  ggplot(aes(x=dose,y=length,fill=dose))+
  geom_boxplot() +
  geom_point(position="jitter")

dna %>%
  ggplot(aes(sample=length)) +
  geom_qq() + 
  geom_qq_line() +
  facet_wrap(~dose)
```

- Strong indication that data in control group has a lower variance.
- 6 observations per group are too few to check the assumptions 

```{r}
plot(lm(length~dose,data=dna))
```

# Kruskal-Wallis Rank Test

- The Kruskal-Wallis Rank Test (KW-test) is a  non-parameteric alternative  for ANOVA F-test.  
  
-  Classical $F$-teststatistic can be written as
  \[
    F = \frac{\text{SST}/(g-1)}{\text{SSE}/(n-g)} = \frac{\text{SST}/(g-1)}{(\text{SSTot}-\text{SST})/(n-g)} ,
  \]
-  with $g$ the number of groups. 
 
- SSTot depends only on  outcomes $\mathbf{y}$ and will not vary in permutation test. 

- SST can be used as statistic
 $$\text{SST}=\sum_{j=1}^t n_j(\bar{Y}_j-\bar{Y})^2$$


-  The KW test statistic is based on SST on rank-transformed outcomes^[we assume that no *ties* are available],
  \[
     \text{SST} = \sum_{j=1}^g n_j \left(\bar{R}_j - \bar{R}\right)^2 = \sum_{j=1}^t n_j \left(\bar{R}_j - \frac{n+1}{2}\right)^2 ,
  \]
-  with $\bar{R}_j$ the mean of the ranks in group $j$, and $\bar{R}$ the mean of all ranks,
  \[
    \bar{R} = \frac{1}{n}(1+2+\cdots + n) = \frac{1}{n}\frac{1}{2}n(n+1) = \frac{n+1}{2}.
  \]
-  The KW teststatistic is given by
  \[
    KW = \frac{12}{n(n+1)}  \sum_{j=1}^g n_j \left(\bar{R}_j - \frac{n+1}{2}\right)^2.
  \]
-  The factor $\frac{12}{n(n+1)}$ is used so that $KW$ has a simple asymptotic null distribution. In particular under $H_0$, given thart $\min(n_1,\ldots, n_g)\rightarrow \infty$, 
  \[
    KW  \rightarrow \chi^2_{t-1}.
  \]

-  The exact KW-test can be executed by calculating the permutation null distribution (that only depends on $n_1, \ldots, n_g$) to test
  $$H_0: f_1=\ldots=f_g \text{ vs } H_1: \text{ at least two means are different}.$$ 
  
- In order to allow $H_1$ to be formulated in terms of means, the assumption of locations shift should be valid.
- For DMH example this is not the case.   
- If location-shift is invalid, we have to formulate $H_1$ in terms of probabilistic indices:
  $$H_0: f_1=\ldots=f_g \text{ vs } H_1: \exists\ j,k \in \{1,\ldots,g\} : \text{P}\left[Y_j\geq Y_k\right]\neq 0.5$$


## DNA Damage Example

```{r}
kruskal.test(length~dose,data=dna)
```

- On the $5\%$ level of significance we can reject the null hypothesis. 
  
- R-functie `kruskal.test` only returns the asymptotic approximation for $p$-values. 

- With only 6 observaties per groep, this is not a good approximation of the $p$-value

-  With the `coin` R package we can calculate the exacte $p$-value

```{r,warning=FALSE,message=FALSE}
library(coin)
kwPerm <- kruskal_test(length~dose,data=dna,
		    distribution=approximate(B=100000))
kwPerm
```

- We conclude that the difference in the distribution of the DNA damages due to the DMH dose is extremely significantly different. 

- Posthoc analysis with WMW tests. 

```{r}
pairwise.wilcox.test(dna$length,dna$dose)
```

- All DMH behandelingen are significantly different from the control. 
- The DMH are not significantly different from one another.  
- U1 does not occur in the `pairwise.wilcox.test` output. Point estimate on probability on higher DNA-damage?

```{r, echo=FALSE}
pairWilcox <- pairwise.wilcox.test(dna$length,dna$dose)
```

```{r}
nGroup <- table(dna$dose)
probInd <- combn(levels(dna$dose),2,function(x) 
		 {
		 test <- wilcox.test(length~dose,subset(dna,dose%in%x))
		 return(test$statistic/prod(nGroup[x]))
		 }
		 )
names(probInd) <- combn(levels(dna$dose),2,paste,collapse="vs")
probInd
```

Because there are doubts on the location-shift model we draw our conclusions in terms of the probabilistic index. 

### Conclusion

- There is an extremely significant difference in in the distribution of the DNA-damage measurements due to the treatment with DMH  ($p<0.001$ KW-test).
- DNA-damage is more likely upon DMH treatment than in the control treatment (all p=0.013, WMW-testen). 
- The probability on higher DNA-damage upon exposure to DMH is 100% (Calculation of a CI on the probabilistic index is beyond the scope of the course) 
- There are no significant differences in the distributions of the comit-lengths among the treatment with the different DMH concentrations ($p=$ `r paste(format(range(pairWilcox$p.value[,-1],na.rm=TRUE),digit=2),collapse="-")`). 
- DMH shows already genotoxic effects at low dose. 
- (Alle paarswise tests are gecorrected for multiple testing using Holm's methode).

